Effect of exercise on estrogen deficiency-induced depression-like behavior and its inflammatory mechanisms / 第二军医大学学报
Academic Journal of Second Military Medical University
; (12): 477-482, 2015.
Article
em Zh
| WPRIM
| ID: wpr-838914
Biblioteca responsável:
WPRO
ABSTRACT
Objective To explore whether exercise training can ameliorate estrogen deficiency-induced depression-like behavior and its mechanisms, so as to provide evidence for treating menopausal depression with exercise. Methods A total of 60 female adult C57BL/6J mice were randomly assigned to Sham-sedentary (Sham), Sham-exercise (Sham+Ex), Ovx-sedentary (Ovx) and Ovx-exercise (Ovx+Ex) groups. Sham operation or bilateral Ovx was performed. After recovery for 1 week, Sham+ Ex and Ovx+Ex mice were adapted to the treadmill for 5 weeks. We use sucrose consumption test, open field test and tail suspension test to inspect the mice of behavior change; then the mice were decapitated, and the serum and hippocampus samples were collected. 5-hydroxytryptamine (5-HT), indoleamine 2, 3-dioxygenase (IDO) and inflammatory cytokine in the hippocampus were determined using a commercial ELISA kit. Results Compared with Sham mice, Ovx mice developed depression-like behaviors, including loss of interests, decreased exploratory behavior and increased behavioral despair, and exercise training could ameliorate these depression-like behaviors. Compared with the Sham group, Ovx group had significantly decreased hippocampal 5-HT level (P<0. 01) and significantly increased IDO level (P<0. 05) in the hippocampus, which were reversed by exercise training. The hippocampal levels of proinflammatory cytokines, IL-1" (P<0. 05), IL-6 (P<0. 01) and IFN-y (P<0. 01) in Ovx group were significantly increased compared to Sham mice, while the anti-inflammatory cytokines, IL-4 (P<0. 01) and IL-10 (P<0. 05), were significantly decreased in Ovx mice; exercise training could also reverse the alterations of IL1", IFN-y and IL-4 levels in Ovx mice. Conclusion Exercise training can ameliorate estrogen deficiency-induced depression-like behaviors, which may be through improving inflammation in the hippocampus.
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Idioma:
Zh
Revista:
Academic Journal of Second Military Medical University
Ano de publicação:
2015
Tipo de documento:
Article