Changes of Th1/Th2 balance in multiple organ dysfunction syndrome / 第二军医大学学报
Academic Journal of Second Military Medical University
; (12): 1347-1350, 2010.
Article
em Zh
| WPRIM
| ID: wpr-840992
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To explore the pathogenesis of multiple organ dysfunction syndrome (MODS) with respect to the balance of Th1/Th2.Methods:
Eighteen healthy male minipigs, weighing 22-30 kg, were randomly divided into two groups MODS group and control group. Double-hit method including hemorrhagic shock and endotoxiemia was used to establish the porcine MODS model. The peripheral vein blood samples were collected at different time-points (before bloodletting, before endotoxin injection, 1 h, 24 h,48 h and 72 h after endotoxin injection) in the two groups. The spleen samples were collected after death of the animals. Plasma levels of IFN-γ and IL-4 were detected by enzyme-linked immunosorbent assay (ELISA). Real-time PCR was used to detect the expression of IFN-γ, IL-4, T-bet and GATA-3 mRNA (The latter 2 were the key transcription factors associated with Th1/Th2 response) in the spleen samples.Results:
The plasma levels of IFN-γ and IL-4 quickly reached the peak values 1 h after the endotoxin injection, then the level of IFN-γ decreased quickly. The ratio of IFN-γ/IL-4 was significantly lower than the baseline value 72 h after endotoxin injection(P = 0.000). The ratio of IFN-γ/IL-4 mRNA in MODS group was obviously lower than that in the control group (P = 0.020); the ratio of T-bet/GATA-3 was also lower in MODS group (P = 0.038).Conclusion:
The shift from Th1 to Th2 occurs in the progress of MODS.
Texto completo:
1
Índice:
WPRIM
Tipo de estudo:
Prognostic_studies
Idioma:
Zh
Revista:
Academic Journal of Second Military Medical University
Ano de publicação:
2010
Tipo de documento:
Article