Application of FISH(Fluorescence In Situ Hybridization) in uncultured chorionic villus cells / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
; : 248-253, 2000.
Article
em Ko
| WPRIM
| ID: wpr-84909
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE:
The cytogenetic analysis for earlier detection of fetal chromosome aneuploidies is performed from chorionic villus using either long-term culture or direct chromosome preparation. To analyze the cause of pregnancy loss, we also attempt the cytogenetic study in product of conception(POC) using chorionic villi or fetal tissue. But the failure of analysis often occurs in direct preparation of villus cells and product of conception(POC). We studied to evaluate the clinical usefulness of FISH in uncultured chorionic villus cells of culture-failed cases.METHODS:
According to the patient's indication, we performed FISH for chromosome 18, 21, X and Y in chorionic villi as well as POC and compared FISH results with their chromosomal studies.RESULTS:
We found one trisomy 18 and one trisomy 21 in Chorionic Villus Sampling and one trisomy 18 and one monosomy X(45, X) in POC. The averages for accuracy of FISH were 83-91% and all cases are represented consistent results with their chromosomal studies. Among them, we could analyze using FISH only in 5 cases of culture failure including one case of monosomy X in POC.CONCLUSION:
We could detect aneuploidy with uncultured chorionic villus cells in case of culture failure, using FISH, it may be the potential method to assist the cytogenetic study.Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Trissomia
/
Síndrome de Turner
/
Cromossomos Humanos Par 18
/
Amostra da Vilosidade Coriônica
/
Córion
/
Vilosidades Coriônicas
/
Síndrome de Down
/
Análise Citogenética
/
Citogenética
/
Feto
Limite:
Pregnancy
Idioma:
Ko
Revista:
Korean Journal of Obstetrics and Gynecology
Ano de publicação:
2000
Tipo de documento:
Article