Effects of imatinib on the expression of SHIP gene and apoptosis of K562 cells / 肿瘤
Tumor
; (12): 853-856, 2007.
Article
em Zh
| WPRIM
| ID: wpr-849469
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To observe the changes of SHIP, caspase-1, caspase-3, caspase-9 and bcl-2 gene expression levels in K562 cells after mesylate imatinib (MI) treatment and explore the possible mechanism for apoptosis-inducing effects of imatinib.Methods:
K562 cells were cultured with MI at different concentrations. The cells were collected at different time points. Real-time quantitative PCR was used to detect the expression level of SHIP gene. Semi-quantitative reverse transcriptase PCR was used to detect the mRNA transcription of anti-apoptotic gene bcl-2 and pro-apoptotic genes caspase-1, caspase-3 and caspase-9. The cell proliferation was measured by MTT assay. The cell apoptosis was analyzed by Annexin V/PI double staining flow cytometry.Results:
MI significantly increased the expression of SHIP gene in a time-and dose-dependent manner. Caspase-9 gene was also up-regulated after MI treatment and had linear correlation with SHIP gene expression. The expression levels of caspase-1, caspase-3 and bcl-2 had no significant changes. MI down-regulated the proliferation and induced the apoptosis of K562 cells. The morphological changes of typical of apoptosis were observed.Conclusion:
MI significantly increases the expressions of SHIP gene and caspase-9 gene and induces apoptosis of K562 cells. The mechanism for the apoptosis-inducing effects of imatinib may be associated with the up-regulation of SHIP and caspase-9 gene.
Texto completo:
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Índice:
WPRIM
Idioma:
Zh
Revista:
Tumor
Ano de publicação:
2007
Tipo de documento:
Article