Experimental studies on anti-fatigue effects and spectra-effect relationship of different extracts from black Lepidium meyenii (Maca) / 中草药
Chinese Traditional and Herbal Drugs
; (24): 2090-2096, 2018.
Article
em Zh
| WPRIM
| ID: wpr-852004
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ABSTRACT
Objective To investigate the anti-fatigue effects and the relationship between UPLC and anti-fatigue effects of different extracts of black Maca, and to provide a basis for clarifying the material basis of anti-fatigue effects of black Maca. Methods Anti- fatigue effects of eight different extracts of black Maca were evaluated through exhaustive swimming time, contents of liver glycogen, and lactic acid in serum; UPLC-Q-TOF/MS was applied to establish the fingerprints for black Maca from eight extracts; Using the anti-fatigue effects of exhaustive swimming experiment as pharmacodynamic indicators, spectra-effect relationship was analyzed by using PLSR. Results 60%, 80%, and 95% ethanol extracts of black Maca could significantly prolong the exhaustive swimming time of mice, with the effects of 95% ethanol extract of black Maca was the strongest; The treatment of 80% ethanol extract of black Maca significantly increased the level of depressed hepatic glycogen due to excessive exercise; Moreover, 95% ethanol extract of black Maca substantially decreased the serum lactic acid accumulation after loaded-swimming. A total of 23 characteristic peaks were characterized by HPLC fingerprints of eight different extracts of black Maca. N-benzylhexadecanamide, N-benzyl-5-oxo-6E,8E-octadecadienamide, 1,3-dibenzyl-2-phenyl-4,5-dimethylimidazilium, and N-octadecanamide were found to be positively related to the anti-fatigue effects with VIP > 1 in extracts of black Maca from PLSR analysis by using anti-fatigue effects of exhaustive swimming time as pharmacodynamic indicators. Conclusion 95% and 80% ethanol extracts of black Maca showed the obvious anti-fatigue function. It is clear that four components N-benzylhexadecanamide, N-benzyl-5-oxo-6E,8E-octadecadienamide, 1,3-dibenzyl-2-phenyl-4,5-dimethylimidazilium, and N-octadecanamide are the principal anti-fatigue substances in black Maca. The study has the contribution to to explore the material basis of anti-fatigue effects and provides new ideas for the omprehensive and reliable quality control of black Maca.
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Zh
Revista:
Chinese Traditional and Herbal Drugs
Ano de publicação:
2018
Tipo de documento:
Article