Icaritin exerted effect in anti-postmenopausal osteoporosis by binding to RANKL protein target and inhibiting bone resorption / 中草药

ABSTRACT

Objective To demonstrate that icaritin (IT) inhibits bone resorption against osteoporosis by binding to RANKL protein targets. Methods The effects of RANKL and IT on the osteoporosis of ovariectomized rats were established by molecular docking technique. The effects of IT on the body weight, bone resorption serum index (ALP and TRACP-5b), bone mineral density and bone morphology of OVX rats were evaluated. Results IT could be stably docked with target protein RANKL. The body weight of IT group was significantly lower than that of OVX group (P andlt; 0.05). IT could significantly decrease the levels of serum ALP and TRACP-5b (P andlt; 0.01), and the value of femur BS/BV and Tb.Sp (P andlt; 0.01) in OVX rats. Moreover, IT significantly increased BMD, BV/TV, Tb.ThN, Tb.N values (P andlt; 0.01). Conclusion IT can inhibit osteoclast differentiation and play anti-osteoporosis by binding with RANKL protein target.