Mechanism of loropetalum chinensis in treatment of diabetic skin ulcer / 中草药

ABSTRACT

Objective To study the molecule mechanism of ethanol extract of Loropetalum chinensis (EELC) in the treatment of diabetic skin ulcer. Methods In vitro epidermal stem cell (ESCs) culture technique and dual luciferase reporter gene detection system were used to study the mechanism of EELC in the treatment of diabetic skin ulcer. Results Totally 232.8 g powder was extracted from 4.5 kg Loropetalum chinensis by 75% ethanol, and the main ingredient was polyphenol. After treating for 48 h with different doses of EELC (50 and 100 μg/mL), the expression of WNT protein, β-catenin protein and C-myc protein was all up-regulated, and was positively correlated with the dose effect (P < 0.01). The target effect of EELC on the Wnt signaling pathway was further identified by the dual luciferase reporter gene assay system. Conclusion The results suggested that EELC could activate canonical Wnt signaling pathway, promote β-catenin protein accumulation in combination with transcription factor in the nucleus to activate the expression of specific target genes, promote ESCs proliferation and differentiation, and repair skin wounds of diabetic ulcers effectively as well.