Determination of multiple functional substances in different processed products of Polygoni Multiflori Radix and principal component analysis / 中草药

ABSTRACT

Objective: To study the effect of different processing methods and conditions for bioactive constituents in Polygoni Multiflori Radix (the root tubers of Polygonum multiflorum) and their change regularity, in order to optimize and establish appropriate drying methods and conditions. Methods: The stilbene glucoside, free-anthraxquinones, combined-anthraxquinones, and catechin from 12 kinds of Polygoni Multiflori Radix in different processing methods were determined by MEKC-DAD. The multiple functional substances of principal component analysis (PCA) was made to standardized treatment for the comprehensive evaluation of the 12 samples by different drying methods. Results: The contents of multiple functional substances in different processed products from Polygoni Multiflori Radix presented the certain regularity. The contents of stilbene glucoside and combined anthraquinone were the highest in Polygoni Multiflori Radix which was cut into thick pieces dried in the sun, the total free anthraquinones were the highest in Polygoni Multiflori Radix which was cut into thick pieces dried at 70℃, the content of catechin was the highest in Polygoni Multiflori Radix which was cut into thin pieces dried at 40℃. The comprehensive evaluation index obtained with PCA showed that the sample cut into thick pieces dried in the sun was significantly higher than those with other samples. Conclusion: The influence of processing methods by the determination of multiple functional substances in Polygoni Multiflori Radix has been investigated and a scientific basis for the suitable processing method of Polygoni Multiflori Radix provided.