Content changes of chemical constituents in Ginseng Radix and Atractylodis Macrocephalae Rhizoma before and after compatibility / 中草药

ABSTRACT

Objective: To discuss the synergistic mechanism of compatibility of Ginseng Radix (GR) and Atractylodis Macrocephalae Rhizoma (AMR), and to determine the compounds in GR and AMR before and after compatibility using HPLC method. Methods: Hypersil ODS column (250 mm × 4.6 mm, 5 μm) was adopted. The mobile phase was acetonitrile (A) and water (B) for gradient elution (0-35 min, 5%-35% A; 35-40 min, 35% A; 40-80 min, 35%-95% A). The detection wavelength was set at 203 nm. The column temperature was 25℃. The flow rate was 1 mL/min. Results: The HPLC analysis suggested the extracts before and after compatibility were different on contents of GR and AMR. In GR and AMR co-decoction, the contents of ginsenosides Rg1, Re, Rf, Rd, and butenolide I increased obviously, and a new chromatographic peak appeared. There was no obvious change in other components. Conclusion: The synergistic mechanism of the compatibility of GR and AMR can be proved with the increased dissolution of ginsenoside and a new chromatographic peak.