Effect of plant growth regulators on major secondary metabolite accumulation in callus of Taxus cuspidata / 中草药

ABSTRACT

Objective: To increase the contents of Taxol and 10-deacetylbaccatin III in the callus of Taxus cuspidata through the application and combination of various plant growth regulators. Methods: Young stems of T. cuspidata were taken as expiants and then their effects of NAA, 2,4-D, and 6-BA on the accumulation of Taxol and 10-deacetylbaccatin III were examined with the method of orthogonal design. Results: NAA exhibited the largest effect on the yields of both total Taxol and 10-deacetylbaccatin III, and followed by 6-BA, 2,4-D. Conclusion: The optimal medium for production of total Taxol and total 10-deacetylbaccatin III is B5+NAA 2.0 mg/L+2,4-D 0.5 mg/L+6-BA 0.2 mg/L.