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Statistical Analysis of Gene Expression in Innate Immune Responses: Dynamic Interactions between MicroRNA and Signaling Molecules
Genomics & Informatics ; : 107-112, 2007.
Article em En | WPRIM | ID: wpr-86066
Biblioteca responsável: WPRO
ABSTRACT
MicroRNAs (miRNAs) are known to negatively control protein-coding genes by binding to messenger RNA (mRNA) in the cytoplasm. In innate immunity, the role of miRNA gene silencing is largely unknown. In this study, we performed microarray-based experiments using lipopolysaccharide (LPS)-stimulated macrophages derived from wild-type, MyD88 knockout (KO), TRIF KO, and MyD88/TRIF double KO mice. We employed a statistical approach to determine the importance of the commonality and specificity of miRNA binding sites among groups of temporally co-regulated genes. We demonstrate that both commonality and specificity are irrelevant to define a priori groups of co-downregulated genes. In addition, analyzing the various experimental conditions, we suggest that miRNA regulation may not only be a late-phase process (after transcription) but can also occur even early (1h) after stimulation in knockout conditions. This further indicates the existence of dynamic interactions between miRNA and signaling molecules/ transcription factor regulation; this is another proof for the need of shifting from a 'hard-wired' paradigm of gene regulation to a dynamical one in which the gene co-regulation is established on a case-by-case basis.
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Texto completo: 1 Índice: WPRIM Assunto principal: Fatores de Transcrição / Sítios de Ligação / RNA Mensageiro / Expressão Gênica / Sensibilidade e Especificidade / Inativação Gênica / Citoplasma / MicroRNAs / Imunidade Inata / Macrófagos Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Genomics & Informatics Ano de publicação: 2007 Tipo de documento: Article
Texto completo: 1 Índice: WPRIM Assunto principal: Fatores de Transcrição / Sítios de Ligação / RNA Mensageiro / Expressão Gênica / Sensibilidade e Especificidade / Inativação Gênica / Citoplasma / MicroRNAs / Imunidade Inata / Macrófagos Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: Genomics & Informatics Ano de publicação: 2007 Tipo de documento: Article