Phase-changeable nanoparticles loaded with FeIII-tannic acid/paclitaxel for in vitro ultrasound imaging and treatment of retinoblastoma / 中国医学影像技术

ABSTRACT

Objective: To prepare nanoparticles able to be used in early diagnosis and treatment of retinoblastoma (Rb), and to evaluate the in vitro ultrasound imaging capability and photothermal effect. Methods: Nanoparticles containing poly(lactic-co-glycolic acid) (PLGA) shell, FeIII-tannic acid (FeIIITA), paclitaxel (PTX) and perflenapent (PFP) were prepared by double emulsification. The particle size, potential, encapsulation efficiency and drug loading rate were measured. The temperature and ultrasound signal change were evaluated after 808 nm near-infrared laser (1 W/cm2, 5 min) irradiation, and the phase transition of nanoparticles was observed under light microscope. Moreover, the biosafety of FeIIITA/PLGA/PFP nanoparticles was assessed in vitro. The following experimental groups were established according to different types of nanoparticles, i.e. control group, laser group, FeIIITA/PLGA/PTX/PFP (FPTP) group, FeIIITA/PLGA/PTX/PFP+laser (FPTP+laser) group as well as different concentrations of nanoparticles (0, 0.250, 0.500, 1.000 g/L) in FPTP+laser group. The killing effects on Y79 cells in all aforementioned groups were evaluated after irradiation for 5 minutes. Results: FeIIITA/PLGA/PTX/PFP nanoparticles were successfully prepared, the average particle size of nanoparticles was (155.8±55.68) nm and the mean surface charge was (-27.3±5.14) mV. Gray core with black FeIIITA coated nanoparticles could be seen under transmission electron microscope. The encapsulation efficiency and drug loading rate of particles was (70.89±8.03)% and (9.61±0.63)%, respectively. In addition, temperature increased when applying irradiation in vitro and positively correlated with the concentration of nanoparticles. Moreover, the ultrasonic signals in B-mode and imaging-mode enhanced with increasing concentration of nanoparticles. The killing effect against Y79 in FPTP+laser group was stronger than that of control group, laser group and FTTP group in vitro, positively correlated with the concentration of nanoparticles. Conclusion: The phase changeable nanoparticles loaded with FeIII-tannic acid and PFP were successfully prepared and possessed excellent photothermal effect and ultrasound imaging effect along with a robust killing effect on Y79 cells, being expected to realize the integrated approach in diagnosis and treatment of Rb.