Effects of exercise on the celluar immune function of mice induced by PM2.5 / 上海预防医学

ABSTRACT

Objective To explore the effect of exercise on the celluar immune function of mice induced by PM2.5. Methods Twenty-four male C57BL/6 mice were randomly assigned into four groups, filtered air, concentrated PM2.5, exercise and filtered air, and exercise and concentrated PM2.5, with six mice in each group.The mice in the exercise group ran on the treadmill for 1 h every day, and then the mice in the four groups were respectively exposed to concentrated PM2.5 or filtered air after 1 h of rest.After twenty-four weeks, flow cytometric assay was used to detect the quantity of CD3+CD4+CD8a+ T cells, CD18+ T cells and CD154+ T cells in spleen in mice of the four groups. Results The median concentration of PM2.5 in the exposure chamber, filtered chamber and ambient air were 49.24, 12.18, 32.25 μg/m3, respectively.PM2.5 exposure led to increase in CD3+CD4+CD8a+ T cells (P < 0.05) and decrease in CD154+ T cells (P < 0.05), whereas running was beneficial for decreasing CD3+CD4+CD8a+ T cells (P < 0.05) and increasing CD154+ T cells (P < 0.05). Conclusions PM2.5 exposure induces perturbation of the immune system.Regular running proves to be helpful for maintaining the balance of the immune system and improving the body′s resistance to PM2.5.