Nuclear Factor-κB Signaling Mediates Antimony-induced Astrocyte Activation / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 29-39, 2021.
Article
em En
| WPRIM
| ID: wpr-878318
Biblioteca responsável:
WPRO
ABSTRACT
Objective@#Antimony (Sb) has recently been identified as a novel nerve poison, although the cellular and molecular mechanisms underlying its neurotoxicity remain unclear. This study aimed to assess the effects of the nuclear factor kappa B (NF-κB) signaling pathway on antimony-induced astrocyte activation.@*Methods@#Protein expression levels were detected by Western blotting. Immunofluorescence, cytoplasmic and nuclear fractions separation were used to assess the distribution of p65. The expression of protein in brain tissue sections was detected by immunohistochemistry. The levels of mRNAs were detected by Quantitative real-time polymerase chain reaction (qRT-PCR) and reverse transcription-polymerase chain reaction (RT-PCR).@*Results@#Antimony exposure triggered astrocyte proliferation and increased the expression of two critical protein markers of reactive astrogliosis, inducible nitric oxide synthase (iNOS) and glial fibrillary acidic protein (GFAP), indicating that antimony induced astrocyte activation @*Conclusion@#Antimony activated astrocytes by activating the NF-κB signaling pathway.
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Índice:
WPRIM
Assunto principal:
Transdução de Sinais
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Astrócitos
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NF-kappa B
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MAP Quinase Quinase Quinases
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Linhagem Celular Tumoral
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Proliferação de Células
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Óxido Nítrico Sintase Tipo II
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Proteína Glial Fibrilar Ácida
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Camundongos Endogâmicos ICR
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Antimônio
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2021
Tipo de documento:
Article