Using dynamic molecular switches for shikimic acid production in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 2104-2112, 2020.
Artigo
em Chinês
| WPRIM
| ID: wpr-878470
ABSTRACT
Shikimic acid is an intermediate metabolite in the synthesis of aromatic amino acids in Escherichia coli and a synthetic precursor of Tamiflu. The biosynthesis of shikimic acid requires blocking the downstream shikimic acid consuming pathway that leads to inefficient production and cell growth inhibition. In this study, a dynamic molecular switch was constructed by using growth phase-dependent promoters and degrons. This dynamic molecular switch was used to uncouple cell growth from shikimic acid synthesis, resulting in the production of 14.33 g/L shikimic acid after 72 h fermentation. These results show that the dynamic molecular switch could redirect the carbon flux by regulating the abundance of target enzymes, for better production.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ácido Chiquímico
/
Microbiologia Industrial
/
Proteínas de Escherichia coli
/
Escherichia coli
/
Engenharia Metabólica
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2020
Tipo de documento:
Artigo
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