Self-assembly in the transparent droplets formed during the screening of protein self-assembly conditions / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1396-1405, 2021.
Article
em Zh
| WPRIM
| ID: wpr-878641
Biblioteca responsável:
WPRO
ABSTRACT
Protein self-assemblies at the micro- and nano-scale are of great interest because of their morphological diversity and good biocompatibility. High-throughput screening of protein self-assembly at different scales and morphologies using protein crystallization screening conditions is an emerging method. When using this method to screen protein self-assembly conditions, some apparently transparent droplets are often observed, in which it is not clear whether self-assembly occurs. We explored the interaction between β-lactoglobulin and the protein crystallization kit Index™ C10 and observed the presence of micro- and nano-scale protein self-assemblies in the transparent droplets. The diverse morphology of the micro- and nano-scale self-assemblies in the transparent droplets formed by mixing different initial concentrations of β-lactoglobulin and Index™ C10 was further investigated by scanning electron microscope. Self-assembly process of fluorescence-labelled β-lactoglobulin was monitored continuously by laser confocal microscope, allowing real-time observation of the liquid-liquid phase separation phenomenon and the morphology of the final self-assemblies. The internal structure of the self-assemblies was gradually ordered over time by in-situ X-ray diffraction. This indicates that the self-assembly phenomenon within transparent droplets, observed in protein self-assembly condition screening experiments, is worthy of further in-depth exploration.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Cristalização
/
Lactoglobulinas
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2021
Tipo de documento:
Article