Prenatal diagnosis of spinal muscular atrophy using multiple ligation-dependent probe amplification / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 214-218, 2021.
Article
em Zh
| WPRIM
| ID: wpr-879556
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To carry out prenatal diagnosis for families with high risk for spinal muscular atrophy (SMA) by using multiplex ligation-dependent probe amplification (MLPA).@*METHODS@#Twenty-one families were enrolled. MLPA was used to detect copy numbers of SMN1 and SMN2 genes. Maternal contamination was excluded by using a short tandem repeat method.@*RESULTS@#For 23 fetuses from the 21 families, 14 were identified as carriers, 1 as SMA patient, and 8 as normal. By linkage analysis of parental samples, three individuals were determined as silent (2+0) carriers.@*CONCLUSION@#MLPA can determine the carrier status of SMA. The identification of three silent (2+0) carriers among the 44 parental samples indicated a risk for such families, for which genetic counseling and reproduction guidance should be provided.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Diagnóstico Pré-Natal
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Atrofia Muscular Espinal
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Proteína 1 de Sobrevivência do Neurônio Motor
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Reação em Cadeia da Polimerase Multiplex
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Aconselhamento Genético
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Heterozigoto
Tipo de estudo:
Diagnostic_studies
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Guideline
Limite:
Female
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Humans
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Pregnancy
Idioma:
Zh
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2021
Tipo de documento:
Article