Inhibitory Effects of Agaricus Blazei Murrill (FA-2-b-β) on Proliferation of Chronic Myeloid Leukemia Cells in vivo and Its Mechanism / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1885-1891, 2020.
Article
em Zh
| WPRIM
| ID: wpr-879988
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To investigated the anti-tumor in vivo effect and mechanism of the acid RNA protein complex (FA-2-b-β) of Agaricus blazei Murrill extract.@*METHODS@#CCK-8 method was used to detected the inhibitory effect of FA-2-b-β on proliferation of primary CML cells from newly diagnosed CML patients, the CML mouse model was established by trail-venous injection of primary CML cells, and the survival time, blood cell count and body weight were observed, the immunoflouresence and immunehistochemistry analysis, RT-qPCR, Western bolt were used to detemine the expression of caspase-3 signal pathway-related apoptosis genes and proteins.@*RESULTS@#The experiments in vitro showed that the proliferative inhibitory rate in drug-treated group increased with concentration- and time-dependent manner (r@*CONCLUSION@#The FA-2-b-β can induce apoptosis of primary CML cells and prolong the survival time of CML model mouse, which may be related with the caspase-3 signal pathway related genes and proteins.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Agaricus
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Leucemia Mielogênica Crônica BCR-ABL Positiva
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Apoptose
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Células K562
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Proliferação de Células
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Mesilato de Imatinib
Limite:
Animals
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Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2020
Tipo de documento:
Article