Rapid Internal Control Reference Recombinase-Aided Amplification Assays for EBV and CMV Detection / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 650-655, 2021.
Article
em En
| WPRIM
| ID: wpr-887743
Biblioteca responsável:
WPRO
ABSTRACT
Epstein-Barr virus (EBV) and cytomegalovirus (CMV), two of the most prevalent human herpesviruses, cause a wide spectrum of diseases and symptoms and are associated with serious health problem. In this study, we developed an internal control reference recombinase-aided amplification (ICR-RAA) assay for the rapid detection of EBV and CMV within 30 min. The assay had a sensitivity of 5 and 1 copies/test for EBV and CMV, respectively, with no cross reaction with other pathogens. In comparison with those of the commercial quantitative polymerase chain reaction (qPCR), the sensitivity of the EBV and CMV ICR-RAAs using extracted DNA was 93.33% and 84.84%, respectively; the specificity was 98.75% and 100.00%, respectively; and the Kappa values were 0.930 and 0.892 (
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Texto completo:
1
Índice:
WPRIM
Assunto principal:
DNA Viral
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Infecções por Citomegalovirus
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Herpesvirus Humano 4
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Infecções por Vírus Epstein-Barr
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Técnicas de Amplificação de Ácido Nucleico
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Citomegalovirus
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Recombinases
Tipo de estudo:
Diagnostic_studies
Limite:
Adolescent
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Adult
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Child
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Child, preschool
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Female
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Humans
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Infant
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Male
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Newborn
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2021
Tipo de documento:
Article