Research progress in the rapid preparation of monoclonal antibodies of mouse hybridoma / 生物工程学报
Chinese Journal of Biotechnology
; (12): 2293-2306, 2021.
Article
em Zh
| WPRIM
| ID: wpr-887797
Biblioteca responsável:
WPRO
ABSTRACT
Mouse hybridoma monoclonal antibody is the most commonly used antibody in immunology because of its stable source, easy preparation in later stage and high yield. The traditional time-consuming and laborious hybridoma preparation technology could not meet the growing market demand. In this paper, we describe the rapid preparation techniques involved in antigen design and screening, B cell enrichment and screening, transgenic myeloma cells, fusion technology improvement, positive hybridoma cell screening and rapid detection of monoclonal antibody performance, to provide a reference for rapid preparation of mouse hybridoma monoclonal antibody.
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Texto completo:
1
Índice:
WPRIM
Assunto principal:
Linfócitos B
/
Hibridomas
/
Anticorpos Monoclonais
/
Antígenos
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2021
Tipo de documento:
Article