Genetic Effect Analysis of β-globin Gene 3'UTR+101G>C (HBB:c. *233G>C) Variant / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1271-1274, 2021.
Article
em Zh
| WPRIM
| ID: wpr-888551
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To investigate whether β-globin gene 3'UTR+101G>C (HBBc.*233G>C) variant has genetic effect and provide basis for gene diagnosis and genetic counseling.@*METHOD@#Whole blood cell analysis and capillary zone electrophoresis (CZE) were used to analyze the hematological indexes. The most frequent 23 mutations in southern Chinese individuals were routinely measured by PCR-flow fluorenscence immunmicrobeads assay. Sanger sequencing was used to detect the other variants of β-globin gene (HBB).@*RESULTS@#In 463 cases, a total of 7 cases with HBBc.*233G>C variant were detected, among them 4 cases carried other pathogenic variants of HBB gene (2 cases were in trans, 2 cases were in cis), who had typical hematological characteristics of mild β-thalassemia, and 3 cases also carried abnormal hemoglobin variation, but did not have hematological characteristics of β-thalassemia.@*CONCLUSION@#The study shows that HBBc.*233G > C variant has no obvious genetic effect and should be a benign polymorphism.
Texto completo:
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Índice:
WPRIM
Assunto principal:
Hemoglobinas Anormais
/
Talassemia beta
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Regiões 3' não Traduzidas
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Globinas beta
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Mutação
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Ano de publicação:
2021
Tipo de documento:
Article