7SK truncation at 128-179 nt suppresses embryonic stem cell proliferation / 南方医科大学学报
Journal of Southern Medical University
; (12): 1125-1130, 2021.
Article
em Zh
| WPRIM
| ID: wpr-888696
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To explore the role of small nuclear noncoding RNA 7SK in embryonic stem cell (ESCs) proliferation and the value of 7SK as a target for early diagnosis and treatment for primordial dwarfism (PD).@*METHODS@#ESC line R1 was transfected with the CRISPR/Cas9 system, and sequencing of the PCR product and glycerol gradient analysis were performed to identify novel 7SK deletion mutations. A lentivirus system was used to knock down cyclin-dependent kinase 9 (CDK9) in clones with 7SK deletion mutations, and the effect of CDK9 knockdown on the protein level of cell division cycle 6 (CDC6) was analyzed with Western blotting.@*RESULTS@#We identified a novel deletion mutation of 7SK at 128-179 nt in the ESCs, which resulted in deficiency of cell proliferation. 7SK truncation at 128-179 nt significantly reduced the protein expressions of La-related protein 7 (LARP7) and CDC6.@*CONCLUSIONS@#7SK truncation at 128-179 nt can significantly impair proliferation of ESCs by downregulating CDC6. 7SK is a key regulator of proliferation and mediates the growth of ESCs through a mechanism dependent on CDK9 activity, suggesting the value of 7SK truncation at 128-179 nt as a potential target for early diagnosis and treatment of PD.
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Texto completo:
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Índice:
WPRIM
Assunto principal:
Ribonucleoproteínas
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Fatores de Transcrição
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Proteínas Nucleares
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Células HeLa
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Proteínas de Ligação a RNA
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Proteínas de Ciclo Celular
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Fator B de Elongação Transcricional Positiva
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Proliferação de Células
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Células-Tronco Embrionárias
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RNA Longo não Codificante
Tipo de estudo:
Prognostic_studies
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Screening_studies
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Ano de publicação:
2021
Tipo de documento:
Article