Copy number alteration profiling facilitates differential diagnosis between ossifying fibroma and fibrous dysplasia of the jaws / 国际口腔科学杂志·英文版
International Journal of Oral Science
; (4): 21-21, 2021.
Article
em En
| WPRIM
| ID: wpr-888698
Biblioteca responsável:
WPRO
ABSTRACT
Ossifying fibroma (OF) and fibrous dysplasia (FD) are two fibro-osseous lesions with overlapping clinicopathological features, making diagnosis challenging. In this study, we applied a whole-genome shallow sequencing approach to facilitate differential diagnosis via precise profiling of copy number alterations (CNAs) using minute amounts of DNA extracted from morphologically correlated microdissected tissue samples. Freshly frozen tissue specimens from OF (n = 29) and FD (n = 28) patients were obtained for analysis. Lesion fibrous tissues and surrounding normal tissues were obtained by laser capture microdissection (LCM), with ~30-50 cells (5 000-10 000 µm
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Proteínas Nucleares
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Fibroma Ossificante
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Diagnóstico Diferencial
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Variações do Número de Cópias de DNA
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Displasia Fibrosa Óssea
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Galactosiltransferases
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Arcada Osseodentária
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Recidiva Local de Neoplasia
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
International Journal of Oral Science
Ano de publicação:
2021
Tipo de documento:
Article