Analysis and Evaluation on Leaf Quality of Different Artemisia argyi Germplasm Resources / 中国实验方剂学杂志

ABSTRACT

Objective:To analyze the quality differences among different germplasm resources of <italic>Artemisia argyi </italic>and to screen out the specific germplasm by comprehensively evaluating 14 quality traits of 100 germplasm resources. Method:Germplasm resources of <italic>A. argyi </italic>were collected from all over the country. The output rate of moxa and the content of total volatile oil in <italic>A. argyi</italic> leaves were determined，and the contents of 12 flavonoids and phenolic acids in<italic> A. argyi </italic>leaves were detected by ultra performance liquid chromatography（UPLC）. The correlation analysis，principal component analysis and clustering analysis were used to comprehensively evaluate the quality of <italic>A. argyi</italic>. Result:There was rich genetic diversity of<italic> A. argyi</italic> germplasm resources，and the variation coefficients of 14 quality traits ranged from 25.67% to 127.34%，among which the coefficient of variation of chlorogenic acid，cryptochlorogenic acid，isoxiafotaside，isochlorogenic acid B and isochlorogenic acid A was more than 70%，with high variation. The output rate of moxa was negatively correlated with 9 quality traits，while the content of total volatile oil was positively correlated with 10 quality traits，and most of the flavonoids and phenolic acids had synergistic effects. 12 flavonoids and phenolic acids were analyzed by principal component analysis，and 4 principal components could be extracted. The highest contents of flavonoids and phenolic acids were found in S98（Hangzhou，Zhejiang province），S84（Longhui county，Shaoyang city，Hunan province），S66（Futian river town，Macheng city，Hubei province），S35 （Balihu town，Qichun county，Huanggang city，Hubei province），and S15 （Fudao town，Tangyin county，Anyang city，Henan province）. The systematic clustering analysis showed that the 100 germplasm could be divided into four groups when the euclidean distance was 8.0，with 90，3，3，3 and 4 accessions in group Ⅰ，Ⅱ，Ⅲ and Ⅳ，respectively. The germplasm resources in group Ⅱ contained the highest content of flavonoids and phenolic acids，the group Ⅲ contained the highest content of total volatile oil and the group Ⅳ contained the highest output rate of moxa. Conclusion:The leaf quality of different <italic>A. argyi </italic>germplasms is different. This study can provide the basis for the quality evaluation and variety breeding of <italic>A. argyi</italic> germplasm resources.