Application of iPDMS protein microarray in screening of tumor-associated antigen autoantibodies / 生物工程学报
Chinese Journal of Biotechnology
; (12): 4075-4082, 2021.
Article
em Zh
| WPRIM
| ID: wpr-921488
Biblioteca responsável:
WPRO
ABSTRACT
The rapid screening of tumor markers is a challenging task for early diagnosis of cancer. This study aims to use highly sensitive chemiluminescent protein microarray technology to efficiently screen a variety of low abundance tumor related markers. A new material, termed integrated polydimethylsiloxane modified silica gel (iPDMS), was obtained by adding a surface polymerization initiator with olefin end to the conventional polydimethylsiloxane, and fixing into the three-dimensional structure of polydimethylsiloxane by thermal crosslinking through silicon hydrogen bonding. In order to make the iPDMS material resistant to non-specific protein adsorption, a poly(OEGMA) polymer brush was synthesized by surface-initiated atom transfer radical polymerization at the active initiation site. Finally, 20 tumor-related antigens were printed into the specific areas of the microarray by high-throughput spray printing technology, and assembled into 48-well detection microtiterplates of the iPDMS microarray. It was found the VEGFR and VEGF121 autoantibodies that obtained from 8 common tumors (breast cancer, lung cancer, colon cancer, gastric cancer, liver cancer, leukemia, lymphoma and ovarian cancer) can be used as potential tumor markers. The chemiluminescence labeled iPDMS protein microarray can be used for the screening of tumor autoantibodies at early stage.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Autoanticorpos
/
Propriedades de Superfície
/
Adsorção
/
Análise Serial de Proteínas
/
Dimetilpolisiloxanos
/
Sílica Gel
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2021
Tipo de documento:
Article