Effects of Signaling Activation of Bone Morphogenetic Protein 2 on the Differentiation and Infiltration of Regulatory T Lymphocytes in Tumors / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 897-904, 2021.
Article
em Zh
| WPRIM
| ID: wpr-921557
Biblioteca responsável:
WPRO
ABSTRACT
Objective To determine whether the signaling activation of bone morphogenetic protein 2(BMP2)can induce myeloid-derived suppressor cells(MDSC)to secret transforming growth factor β(TGF-β),further enhancing the differentiation and infiltration of regulatory T lymphocytes(Treg)into tumor tissue. Methods The BMP2-induced mRNA and protein expression of TGF-β in MDSC was detected by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay(ELISA),respectively.The effect of BMP2-induced TGF-β secretion by MDSC on Treg differentiation was then determined by flow cytometry.Finally,we implanted the recombined human bone morphogenetic protein 2(rhBMP2)collagen gels into tumor-burdened mice to examine the role of BMP2 in Treg differentiation via MDSC-secreted TGF-β
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Diferenciação Celular
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Fator de Crescimento Transformador beta
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Linfócitos T Reguladores
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Proteína Morfogenética Óssea 2
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Células Supressoras Mieloides
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Neoplasias
Limite:
Animals
Idioma:
Zh
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2021
Tipo de documento:
Article