Application of Nanopore Sequencing Technology in the Clinical Diagnosis of Infectious Diseases / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 381-392, 2022.
Article
em En
| WPRIM
| ID: wpr-927677
Biblioteca responsável:
WPRO
ABSTRACT
Infectious diseases are an enormous public health burden and a growing threat to human health worldwide. Emerging or classic recurrent pathogens, or pathogens with resistant traits, challenge our ability to diagnose and control infectious diseases. Nanopore sequencing technology has the potential to enhance our ability to diagnose, interrogate, and track infectious diseases due to the unrestricted read length and system portability. This review focuses on the application of nanopore sequencing technology in the clinical diagnosis of infectious diseases and includes the following (i) a brief introduction to nanopore sequencing technology and Oxford Nanopore Technologies (ONT) sequencing platforms; (ii) strategies for nanopore-based sequencing technologies; and (iii) applications of nanopore sequencing technology in monitoring emerging pathogenic microorganisms, molecular detection of clinically relevant drug-resistance genes, and characterization of disease-related microbial communities. Finally, we discuss the current challenges, potential opportunities, and future outlook for applying nanopore sequencing technology in the diagnosis of infectious diseases.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Tecnologia
/
Doenças Transmissíveis
/
Análise de Sequência de DNA
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Sequenciamento de Nucleotídeos em Larga Escala
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Microbiota
/
Sequenciamento por Nanoporos
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2022
Tipo de documento:
Article