Identification of chemical constituents in Zhachong Shisanwei Pills by UPLC-Q-TOF-MS/MS / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1546-1557, 2022.
Article
em Zh
| WPRIM
| ID: wpr-928083
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WPRO
ABSTRACT
Zhachong Shisanwei Pills, composed of 13 Chinese medicinal materials, are used for treating the diseases such as hemiplegia, pain of muscles and bones, rheumatism, and joint pain. The chemical composition and pharmacodynamics of Zhachong Shisanwei Pills have not been reported. Ultra-performance liquid chromatography/quadrupole-time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS/MS) was employed to quickly identify the chemical components of Zhachong Shisanwei Pills, which was performed with Shim-pack GIST C_(18) column(4.6 mm×150 mm, 5 μm). The gradient elution was conducted with methanol-0.05% acetic acid as the mobile phase. Electrospray ionization mass spectrometry(ESI-MS) was carried out in both positive and negative ion modes. The compounds were identidied based on accurate relative molecular weight, fragment ion species, and the MS data of reference substances and in literature. In conclusion, a total of 98 compounds were identified, including 19 organic acids, 36 flavonoids, 13 volatile oils, 8 tannins, 5 2-(2-phenylethyl)chromones, 5 amino acids, 3 sesquiterpenoids, 3 alkaloids, and 2 other compounds. This study characte-rized the chemical components of Zhachong Shisanwei Pills rapidly for the first time, laying a foundation for further research on the pharmacodynamic material basis and quality evaluation.
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Assunto principal:
Medicamentos de Ervas Chinesas
/
Cromatografia Líquida de Alta Pressão
/
Cromatografia Líquida
/
Espectrometria de Massas por Ionização por Electrospray
/
Espectrometria de Massas em Tandem
Tipo de estudo:
Diagnostic_studies
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2022
Tipo de documento:
Article