Analysis of Gene Expression in Human Dermal Fibroblasts Treated with Senescence-Modulating COX Inhibitors
Genomics & Informatics
; : 56-64, 2017.
Article
em En
| WPRIM
| ID: wpr-93440
Biblioteca responsável:
WPRO
ABSTRACT
We have previously reported that NS-398, a cyclooxygenase-2 (COX-2)–selective inhibitor, inhibited replicative cellular senescence in human dermal fibroblasts and skin aging in hairless mice. In contrast, celecoxib, another COX-2–selective inhibitor, and aspirin, a non-selective COX inhibitor, accelerated the senescence and aging. To figure out causal factors for the senescence-modulating effect of the inhibitors, we here performed cDNA microarray experiment and subsequent Gene Set Enrichment Analysis. The data showed that several senescence-related gene sets were regulated by the inhibitor treatment. NS-398 up-regulated gene sets involved in the tumor necrosis factor β receptor pathway and the fructose and mannose metabolism, whereas it down-regulated a gene set involved in protein secretion. Celecoxib up-regulated gene sets involved in G2M checkpoint and E2F targets. Aspirin up-regulated the gene set involved in protein secretion, and down-regulated gene sets involved in RNA transcription. These results suggest that COX inhibitors modulate cellular senescence by different mechanisms and will provide useful information to understand senescence-modulating mechanisms of COX inhibitors.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Envelhecimento
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RNA
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Expressão Gênica
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Envelhecimento da Pele
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Aspirina
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Genes vif
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Fator de Necrose Tumoral alfa
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Senescência Celular
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Análise de Sequência com Séries de Oligonucleotídeos
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Ciclo-Oxigenase 2
Limite:
Animals
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Humans
Idioma:
En
Revista:
Genomics & Informatics
Ano de publicação:
2017
Tipo de documento:
Article