Deletion of Salmonella enterica serovar typhimurium sipC gene
Asian Pacific Journal of Tropical Biomedicine
; (12): 987-991, 2015.
Article
em Zh
| WPRIM
| ID: wpr-950860
Biblioteca responsável:
WPRO
ABSTRACT
Objective: To construct a novel plasmid as Salmonella enterica serovar typhimurium (S. typhimurium) sipC gene knockouts candidate. Methods: In this research, 5' upstream and 3' downstream regions of S. typhimurium sipC gene and kanamycin gene were PCR amplified. Each of these DNA fragment was cloned into pGEM T-easy vector. The construct was confirmed by PCR and restriction digest. Results: PCR amplified 320, 206 and 835 bp DNA fragments were subcloned into pET-32 vector resulting with a plasmid called pET-32-sipC up-kan- sip C down. Conclusions: The new plasmid (pET-32-sipC up-kan- sip C down) is useful for genetic engineering and for future manipulation of S. typhimurium sipC gene.
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Índice:
WPRIM
Idioma:
Zh
Revista:
Asian Pacific Journal of Tropical Biomedicine
Ano de publicação:
2015
Tipo de documento:
Article