Recombinant expression and purification of functional vascular endothelial growth factor-121 in the baculovirus expression system
Asian Pacific Journal of Tropical Medicine
; (12): 1195-1199, 2016.
Article
em Zh
| WPRIM
| ID: wpr-951289
Biblioteca responsável:
WPRO
ABSTRACT
Objective To express human vascular endothelial growth factor121 (VEGF121) in insect cells. Methods A gene construct containing VEGF was cloned in the pFastBac-HTA vector, followed by transformation in DH10BAC. The recombinant bacmid was then extracted, and transfected into Sf9 insect cells. The transfected cells were harvested, and then VEGF expression was confirmed by western blotting using specific antibodies. The tube formation assay was used for functional assessment of VEGF. Results Our results showed that VEGF could be successfully expressed in the baculovirus system. Purified VEGF was able to stimulate in vitro tube formation of human endothelial cells. Conclusions Results from this study demonstrated that the recombinantly-produced VEGF can be considered as a promising candidate for therapeutic purposes.
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WPRIM
Idioma:
Zh
Revista:
Asian Pacific Journal of Tropical Medicine
Ano de publicação:
2016
Tipo de documento:
Article