In vivo determination of the gap2 gene promoter activity in Giardia lamblia
The Korean Journal of Parasitology
; : 21-26, 2006.
Article
em En
| WPRIM
| ID: wpr-96037
Biblioteca responsável:
WPRO
ABSTRACT
A shuttle vector for Escherichia coli and Giardia lamblia was modified to produce a reporter plasmid, which monitors the expression of prescribed gene in G. lamblia by measuring its luciferase activity. Promoter regions of the gap2 gene, one of the genes induced during encystation, were cloned into this plasmid, and the resultant constructs were then transfected into trophozoites of G. lamblia. Transgenic trophozoites containing one of the 3 gap2-luc reporters were induced to encystation, and characterized with respect to gap2 gene expression by measuring their luciferase activities. Giardia containing a gap2-luc fusion of 112-bp upstream region showed full induction of luciferase activity during encystation.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Plasmídeos
/
Fatores de Tempo
/
Proteínas Recombinantes de Fusão
/
Transfecção
/
Engenharia Genética
/
Expressão Gênica
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Southern Blotting
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Regiões Promotoras Genéticas
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Giardia lamblia
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Genes de Protozoários
Limite:
Animals
Idioma:
En
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2006
Tipo de documento:
Article