Identification of Novel Genes with Proapoptotic Activity
Genomics & Informatics
; : 77-79, 2006.
Article
em En
| WPRIM
| ID: wpr-96576
Biblioteca responsável:
WPRO
ABSTRACT
In order to identify novel proapoptotic genes, we screened approximately 1,000 hypothetical genes whose functions are completely unknown. After these genes were transiently expressed in HeLa cells, their nuclei images were captured using automated high-speed fluorescence microscope, through which the ratio of apoptotic nuclei was estimated. We selected genes that induce greater than 3-fold increase in apoptotic nuclei compared to that of the vector control. The candidate proapoptotic genes were sequenced and their effects on cell death were further confirmed by the additional assay, DNA fragmentation ELISA. Finally, we were able to identify 4 full-length hypo-thetical genes with proapoptotic activity.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Células HeLa
/
Morte Celular
/
Apoptose
/
Fragmentação do DNA
/
Fluorescência
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Genomics & Informatics
Ano de publicação:
2006
Tipo de documento:
Article