Protection of ischemic preconditioning in the posterior limbs of donor' pancreas graft without involvement adenosine in rat / 中国康复理论与实践
Chinese Journal of Rehabilitation Theory and Practice
; (12): 553-554, 2005.
Article
em Ch
| WPRIM
| ID: wpr-978274
Biblioteca responsável:
WPRO
ABSTRACT
@#ObjectiveTo investigate the effect of ischemic preconditioning (IPC) in the posterior limbs of rats of donor' pancreas graft, and analyze the correlations with adenosine. Methods18 steptozozin (STZ)-induced diabetic SD rats were randomly assigned to 3 groups group I/R (n=6) received pancreas transplantation alone (PTA), Group IPC (n=6) received pancreas transplantation alone exposed IPC with 5 minutes ischemic and 5 minutes reperfusion induced by ligating donor's posterior limb three times before ablating donors, Group IPC+DPCPX received same treatment with Group IPC, but separately injected adenosine A1 receptor antagonist 8-cyclopentyl-1, 3-dipropylxanthine (DPCPX) before IPC. The blood glucose, TNF-α in serum, MDA and MPO in pancreatic tissue were monitored before and after reperfusion, and apoptotice cells were stained by TUNEL technique 2 h after reperfusion.ResultsThe blood glucose, TNF-α, apoptotice index (AI), MDA and MPO of group I/R were higher than that of Group IPC and Group IPC+DPCPX after reperfusion(P<0.01), but those of Group IPC+DPCPX were not markedly different with Group IPC(P>0.05). ConclusionIPC in the posterior limbs of rats can protect rat pancreas graft from I/R injury during PTA. Adenosine do not participate in the signal mechanism of this protection.
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Índice:
WPRIM
Idioma:
Ch
Revista:
Chinese Journal of Rehabilitation Theory and Practice
Ano de publicação:
2005
Tipo de documento:
Article