The expression of autophagy-related proteins in peripheral blood mononuclear cells of patients with lupus nephritis is upregulated and related to kidney damage / 细胞与分子免疫学杂志
Chinese Journal of Cellular and Molecular Immunology
; (12): 633-637, 2023.
Article
em Zh
| WPRIM
| ID: wpr-981910
Biblioteca responsável:
WPRO
ABSTRACT
Objective To identify the relationship between nephritis activity, autophagy and inflammation in patients with SLE. Methods Western blot analysis was used to detect the expression of microtubule-associated protein 1 light chain 3 (LC3) and P62 in peripheral blood mononuclear cells (PBMCs) of SLE patients with lupus nephritis and non-lupus nephritis patients. Tumor necrosis factor α (TNF-α) and interferon γ (IFN-γ) in the serum of SLE patients were determined by ELISA. The correlation between LC3II/LC3I ratio and SLE disease activity score (SLEDAI), urinary protein, TNF-α and IFN-γ levels was analyzed by Pearson method. Results The expression of LC3 was increased and P62 was decreased in SLE patients. TNF-α and IFN-γ were increased in the serum of SLE patients. LC3II/LC3I ratio was positively correlated with SLEDAI (r=0.4560), 24 hour urine protein (r=0.3753), IFN-γ (r=0.5685), but had no correlation with TNF-α (r=0.04 683). Conclusion Autophagy is found in PBMCs of SLE, and the autophagy is correlated with renal damage and inflammation in patients with lupus nephritis.
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Índice:
WPRIM
Assunto principal:
Nefrite Lúpica
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Leucócitos Mononucleares
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Interferon gama
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Fator de Necrose Tumoral alfa
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Proteínas Relacionadas à Autofagia
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Inflamação
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Rim
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Lúpus Eritematoso Sistêmico
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Cellular and Molecular Immunology
Ano de publicação:
2023
Tipo de documento:
Article