The application of PCR-SSCP in forensic mtDNA typing / 法医学杂志
Journal of Forensic Medicine
; (6): 117-119, 2006.
Article
em Zh
| WPRIM
| ID: wpr-983154
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To study the application of PCR-SSCP in forensic mtDNA typing.@*METHODS@#Primers flanking the mtDNA HV-I and HV-II regions were designed. By PCR-SSCP techniques, 70 family trios and 140 unrelated Wuhan Han individuals were investigated and analyzed.@*RESULTS@#In 70 family trios, the SSCP profiles in region HV-I and HV-II of children were not same to that of their fathers in 98.57% and 97.13% respectively but were identical with their mothers. In 140 unrelated Wuhan Han individuals, 21 haplotypes were found in HVI, GD = 0.9556; 16 haplotypes were found in HVII, GD = 0.9356.@*CONCLUSION@#PCR-SSCP technique may be useful in forensic mtDNA typing, especially for screening the suspects.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Linhagem
/
Haplótipos
/
DNA Mitocondrial
/
Reação em Cadeia da Polimerase
/
Impressões Digitais de DNA
/
Análise de Sequência de DNA
/
Primers do DNA
/
Polimorfismo Conformacional de Fita Simples
/
Genética Forense
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Ano de publicação:
2006
Tipo de documento:
Article