Expression of cathepsin-B and -D in rat's brain after traumatic brain injury / 法医学杂志
Journal of Forensic Medicine
; (6): 404-410, 2006.
Article
em Zh
| WPRIM
| ID: wpr-983233
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To study the expression of cathepsin-B and -D in different time point after traumatic brain injury.@*METHODS@#Traumatic brain injury (TBI) model was established on rats, cathepsin-B and cathepsin-D immunofluorescence staining and confocal microscope analysis were performed. Positive cells were counted by confocal microscope and image analysis techniques were used to determine the morphological changes in each group.@*RESULTS@#Immunofluorescence staining results showed that cathepsin-B was activated 1 hour after TBI while cathepsin-D was not activated until 12hour after TBI. Both of them got to their peak during 4 to 8days, and kept a high level of activating 32days after TBI. Cathepsin-B and -D positive cells did not merge with caspase-3 positive cells until 6 h after TBI.@*CONCLUSION@#Cathepsin-B and -D could be the diagnostic markers of TBI and can estimating time course of lateral TBI. They blocked caspase-3 activation at the beginning period after TBI and started to promote cell death with caspase-3 6 h after TBI.
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Índice:
WPRIM
Assunto principal:
Fatores de Tempo
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Encéfalo
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Lesões Encefálicas
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Imuno-Histoquímica
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Catepsina B
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Catepsina D
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Ratos Sprague-Dawley
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Patologia Legal
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Modelos Animais de Doenças
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Caspase 3
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Ano de publicação:
2006
Tipo de documento:
Article