Mutations of short tandem repeat loci in Identifiler system / 法医学杂志
Journal of Forensic Medicine
; (6): 290-294, 2007.
Article
em Zh
| WPRIM
| ID: wpr-983301
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To explore and analyze the mutations of 15 Short Tandem Repeat (STR) loci using Identifiler system in paternity identification.@*METHODS@#2712 cases of paternity testing were carried out using Identifiler PCR Amplification Kit.@*RESULTS@#Of the 2362 paternity testing cases, mutations of single locus were observed in 51 cases. The mutation loci included D8S1179, D21S11, D7S820, CSF1PO, D3S1358, D13S317, D16S539, D2S1338, D19S433, vWA, D18S51, D5S818 and FGA, with the D21S11 locus having a highest mutation rate (0.369%). Thirty-six of the STR mutations were from paternal source, 7 from maternal source, and the rest (9) were undeterminable. The mutation rates at D21S11 were highest (0.369%).@*CONCLUSION@#Mutations of STR loci are relatively common in human genome. Therefore, retesting of additional relatively stable STR loci with lower mutation rates is necessary when one or two loci exclusions are encountered in paternity testing.
Texto completo:
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Índice:
WPRIM
Assunto principal:
Paternidade
/
Análise Mutacional de DNA
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Reação em Cadeia da Polimerase
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Impressões Digitais de DNA
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Sequências de Repetição em Tandem
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Alelos
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Medicina Legal
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Frequência do Gene
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Genética Populacional
/
Mutação
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Ano de publicação:
2007
Tipo de documento:
Article