Analysis of appropriate amount of template DNA for sinofiler kit by real time quantitative PCR technique / 法医学杂志
Journal of Forensic Medicine
; (6): 129-133, 2008.
Article
em Zh
| WPRIM
| ID: wpr-983368
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To explore the appropriate amount of template DNA for Sinofiler Kit.@*METHODS@#The DNA samples with ideally genotyped results by Sinofiler Kit were detected by real-time quantitative PCR assay.@*RESULTS@#It was shown that 1.29-1.51 ng of template DNA in 12.5 microL reaction volume was optimal for STR genotyping with Sinofiler Kit.@*CONCLUSION@#Real time quantitative PCR is an accurate and necessary technique for detection of appropriate amount of template DNA for different kits.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Kit de Reagentes para Diagnóstico
/
Moldes Genéticos
/
DNA
/
Repetições de Microssatélites
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Medicina Legal
/
Cabelo
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Ano de publicação:
2008
Tipo de documento:
Article