The suppression of c-fos in neuron apoptosis induced by heroin / 法医学杂志
Journal of Forensic Medicine
; (6): 321-324, 2010.
Article
em Zh
| WPRIM
| ID: wpr-983586
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To explore the changes of c-fos in apoptosis of cerebellar granular neuron of neonatal SD rats induced by heroin and the mechanisms of neuronal injury caused by heroin.@*METHODS@#Primary cerebellar granular neuron were cultured in vitro, the model of apoptosis induced by heroin was established. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were adopted to investigate the changes of c-fos in cell models.@*RESULTS@#Ten microg/mL of heroin was the optimal dose to induce the apoptosis of cerebellar granular neuron at 48 h. Both Western blotting and RT-PCR showed down regulation of c-fos expression.@*CONCLUSION@#Heroin could induce apoptosis of cerebellar granular neuron and down regulation of c-fos, which may be one of the apoptosis mechanisms.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
RNA Mensageiro
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Regulação para Baixo
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Células Cultivadas
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Cerebelo
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Regulação da Expressão Gênica
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Western Blotting
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Proteínas Proto-Oncogênicas c-fos
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Ratos Sprague-Dawley
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Apoptose
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
Limite:
Animals
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Ano de publicação:
2010
Tipo de documento:
Article