Determination of TTX in biological samples by LC-MS/MS / 法医学杂志
Journal of Forensic Medicine
; (6): 432-435, 2010.
Article
em Zh
| WPRIM
| ID: wpr-983607
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To propose a method for determination of tetrodotoxin (TTX) in human blood, urine and liver by liquid chromatography with tandem mass spectrometry (LC-MS/MS).@*METHODS@#Solid phase extraction is used after the samples are precipitated, then the samples will be analyzed by LC-MS/MS.@*RESULTS@#The limits of detection were 2 ng/mL in blood and urine and 4 ng/g in liver for TIX respectively, the linear correlation coefficients were not less than 0.9973, both of the intra-day and inter-day precisions were less than 12.80%, the recoveries for all kinds of specimens were more than 47.2%.@*CONCLUSION@#This method is efficient, sensitive and accurate and was successfully validated for implementation in forensic toxicological analysis.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Manejo de Espécimes
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Suínos
/
Tetrodotoxina
/
Líquidos Corporais
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Reprodutibilidade dos Testes
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Cromatografia Líquida
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Sensibilidade e Especificidade
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Espectrometria de Massas em Tandem
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Extração em Fase Sólida
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Toxicologia Forense
Limite:
Animals
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Female
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Humans
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Ano de publicação:
2010
Tipo de documento:
Article