Review of Second Generation Sequencing and Its Application in Forensic Genetics / 法医学杂志
Journal of Forensic Medicine
; (6): 282-289, 2016.
Article
em Zh
| WPRIM
| ID: wpr-984849
Biblioteca responsável:
WPRO
ABSTRACT
The rapid development of second generation sequencing (SGS) within the past few years has led to the increasement of data throughput and read length while at the same time brought down substantially the sequencing cost. This made new breakthrough in the area of biology and ushered the forensic genetics into a new era. Based on the history of sequencing application in forensic genetics, this paper reviews the importance of sequencing technologies for genetic marker detection. The application status and potential of SGS in forensic genetics are discussed based on the already explored SGS platforms of Roche, Illumina and Life Technologies. With these platforms, DNA markers (SNP, STR), RNA markers (mRNA, microRNA) and whole mtDNA can be sequenced. However, development and validation of application kits, maturation of analysis software, connection to the existing databases and the possible ethical issues occurred with big data will be the key factors that determine whether this technology can substitute or supplement PCR-CE, the mature technology, and be widely used for cases detection.
Palavras-chave
Texto completo:
1
Índice:
WPRIM
Assunto principal:
DNA Mitocondrial
/
RNA Mensageiro
/
Marcadores Genéticos
/
Reação em Cadeia da Polimerase
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Análise de Sequência de DNA
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Polimorfismo de Nucleotídeo Único
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MicroRNAs
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Genética Forense
Limite:
Humans
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Ano de publicação:
2016
Tipo de documento:
Article