Methylation-Based Age Estimation Model Construction and Its Effectiveness Evaluation / 法医学杂志
Journal of Forensic Medicine
; (6): 17-22, 2019.
Article
em En
| WPRIM
| ID: wpr-984974
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVES@#To screen the DNA methylation loci associated with the age of Han males in northern China and to construct an age estimation model.@*METHODS@#Twenty-one candidate methylation loci were screened. The DNA methylation levels of 476 blood samples from Chinese Han males were detected for 21 amplicons using EpiTYPER technology platform, and data on 153 DNA methylation loci were obtained.@*RESULTS@#After correlation analysis, 8 age-related DNA methylation loci were finally screened. CpG1, CpG2, CpG4, CpG7, CpG8 were located on TRIM59, RASSF5, Clorf132, CSNK1D, ELOVL2,CpG5, CpG6 on PDE4C, and CpG3 on chr1721452808. Based on the 8 loci, 352 samples were used for model construction. A multivariate linear regression age estimation model was constructed (R2=0.93), with mean absolute deviation (MAD) of 2.69 years old. When 109 samples were used for model validation, the MAD was 3.80 years old. The test was repeated 3 times in 15 new samples, with MADs of 4.08, 4.68 and 3.93 years old, respectively.@*CONCLUSIONS@#The age estimation model on Han males in northern China constructed in this study can be used to estimate the age of victims and suspects and to narrow the scope of investigation, and therefore has practical application value.
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Índice:
WPRIM
Assunto principal:
Modelos Lineares
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China
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Ilhas de CpG
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Metilação de DNA
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Proteínas Monoméricas de Ligação ao GTP
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Povo Asiático
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Peptídeos e Proteínas de Sinalização Intracelular
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Proteínas Adaptadoras de Transdução de Sinal
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Proteínas Reguladoras de Apoptose
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Proteínas com Motivo Tripartido
Limite:
Child, preschool
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Humans
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Male
País/Região como assunto:
Asia
Idioma:
En
Revista:
Journal of Forensic Medicine
Ano de publicação:
2019
Tipo de documento:
Article