Method establishment of components identification and content determination of index components in Gantaishu capsules / 中国药房
China Pharmacy
; (12): 2198-2203, 2023.
Article
em Zh
| WPRIM
| ID: wpr-988777
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE To establish methods to identify the chemical components of Gantaishu capsule, and determine the contents of 6 index components including glycyrrhizic acid. METHODS The chemical components of Gantaishu capsule were determined by HPLC-TOF/MS; the contents of 6 index components including glycyrrhizic acid were determined by UPLC-MS/MS. RESULTS A total of 41 chemical components were identified in Gantaishu capsules. The linear ranges of glycyrrhizic acid, mangiferin, luteolin, costunolide, oleanolic acid and berberine were 200-10 000 ng/mL(r were all greater than 0.999). The limits of quantification were 200, 20, 10, 1, 10, 0.5 ng/mL, and the limits of detection were 100, 10, 5, 0.5, 5, 0.25 ng/mL, respectively; RSDs of precision, stability (24 h) and reproducibility tests were all less than 5.0% (n=6 or n=3); the recoveries were 99.05%-101.08% (RSD were all less than 2.0%, n=6). The contents of them were 2.42-2.66, 0.85-1.16, 0.35-0.46, 6.18- 6.46, 0.99-1.29, 5.22-5.56 mg/g. CONCLUSIONS The established methods for identification and content determination are rapid and simple, and can be used for the identification of chemical components and the content determination of index components in Gantaishu capsule.
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WPRIM
Idioma:
Zh
Revista:
China Pharmacy
Ano de publicação:
2023
Tipo de documento:
Article