The application of Compont gel in chronic obstructive jaundice rats model
Acta cir. bras
; 34(5): e201900504, 2019. tab, graf
Article
в En
| LILACS
| ID: biblio-1010871
Ответственная библиотека:
BR1.1
ABSTRACT
Abstract Purpose:
To establish a new rat model, the pathogenesis of which is closer to the clinical occurrence of chronic obstructive jaundice with liver fibrosis.Methods:
90 SD rats were randomly divided into 3 groups. Group A common bile duct ligation, group B common bile duct injection compont and group C injection saline. The serum of three groups was extracted, and the liver function was detected by ELISA. HE staining, Masson staining and immunohistochemistry were used to detect liver pathology.Results:
Group B showed a fluctuant development of jaundice, obstructive degree reached a peak at 2 weeks, and decreased from 3 weeks. HA, LA and PCIII were significantly higher than control group. 3 weeks after surgery, liver tissue fibrosis occurred in group B, and a wide range of fiber spacing was formed at 5 weeks. Immunohistochemistry showed that hepatic stellate cells were more active than the control group.Conclusion:
Intra-biliary injection of Compont gel is different from the classic obstructive jaundice animal model caused by classic bile duct ligation, which can provide an ideal rat model of chronic obstructive jaundice with liver fibrosis.Key words
Полный текст:
1
База данных:
LILACS
Основная тема:
Bile Ducts
/
Disease Models, Animal
/
Gels
/
Liver Cirrhosis
Тип исследования:
Evaluation_studies
/
Prognostic_studies
Пределы темы:
Animals
Язык:
En
Журнал:
Acta cir. bras
Тематика журнала:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Год:
2019
Тип:
Article