Production of dendritic cell vaccines using different methods with equivalent results: Implications for emerging centers
Hematol., Transfus. Cell Ther. (Impr.)
; 46(1): 30-35, Jan.-Mar. 2024. tab, graf
Article
в En
| LILACS
| ID: biblio-1557873
Ответственная библиотека:
BR408.1
Локализация: BR408.1
ABSTRACT
Introduction Dendritic cell (DC) vaccines have demonstrated good efficacy in preventing relapse and in increasing survival of patients affected by a variety of both solid and hematological tumors. Most protocols used to generate these cells involve the automated separation of peripheral blood monocytes from patients. This approach requires specialized equipment, which elevates the cost of this type of therapy, potentially limiting the widespread access to patients. Method:
In this study, we compare the yield and quality of dendritic cells generated from monocytes and isolated by an automated method or by manual methods using gradient centrifugation. Results The results demonstrate the equivalence of the 3 methods in relation to the yield and final quality of the product, however with considerable differences between the costs of these procedures. In addition, this study also demonstrates the feasibility of the antigenic pulse with autologous tumor cell lysates, constituting a source of antigens, not only easily obtained and manipulated, but also specific to the patient's tumor. Conclusion These findings may have important implications for emerging centers interested in using this medical approach and potentially increase the access of a greater number of patients to this therapeutic option.Key words
Полный текст:
1
База данных:
LILACS
Основная тема:
Dendritic Cells
Язык:
En
Журнал:
Hematol Transfus Cell Ther
/
Hematol., Transfus. Cell Ther. (Impr.)
/
Hematology, Transfusion and Cell Therapy (Impresso)
/
Hematology, Transfusion and Cell Therapy (Online)
Тематика журнала:
Hematologia
/
TransfusÆo de Sangue
Год:
2024
Тип:
Article