Perconditioning combined with postconditioning on kidney ischemia and reperfusion
Acta cir. bras
; Acta cir. bras;32(8): 599-606, Aug. 2017. tab, graf
Article
в En
| LILACS
| ID: biblio-886224
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ABSTRACT
Abstract Purpose:
To evaluate if combination of perconditioning and postconditioning provides improved renal protection compared to perconditioning alone in a model of renal reperfusion injury.Methods:
Thirty rats were assigned into 6 groups normality; sham; ischemia and reperfusion; postconditioning; perconditioning; perconditioning + postconditioning. Animals were subjected to right nephrectomy and left renal ischemia for 30 minutes. Postconditioning consisted of 3 cycles of 5 min renal perfusion followed by 5 min of renal ischemia after major ischemic period. Perconditioning consisted of 3 cycles of 5 min hindlimb ischemia followed by 5 min of hindlimb perfusion contemporaneously to renal major ischemic period. After 24 hours, kidney was harvested and blood collected to measure urea and creatinine.Results:
Perconditioning obtained better values for creatinine and urea level than only postconditioning (p<0.01); performing both techniques contemporaneously had no increased results (p>0.05). Regarding tissue structure, perconditioning was the only technique to protect the glomerulus and tubules (p<0.05), while postconditioning protected only the glomerulus (p<0.05). Combination of both techniques shows no effect on glomerulus or tubules (p>0.05).Conclusions:
Perconditioning had promising results on ischemia and reperfusion induced kidney injury, enhanced kidney function and protected glomerulus and tubules. There was no additive protection when postconditioning and perconditioning were combined.Key words
Полный текст:
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База данных:
LILACS
Основная тема:
Reperfusion Injury
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Ischemic Preconditioning
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Ischemic Postconditioning
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Ischemia
/
Kidney
Тип исследования:
Evaluation_studies
/
Prognostic_studies
Пределы темы:
Animals
Язык:
En
Журнал:
Acta Cir Bras
/
Acta cir. bras
/
Acta cir. bras. (Online)
/
Acta cirúrgica brasileira (Impresso)
Тематика журнала:
Cirurgia Geral
/
Procedimentos Cir£rgicos Operat¢rios
Год:
2017
Тип:
Article