Decrease in the ratio of high to low affinity isozymes of (Na+ +K+)-ATPase during the development of rat cardiac ventricles
Braz. j. med. biol. res
; 28(3): 363-7, Mar. 1995. tab, graf
Article
в En
| LILACS
| ID: lil-154705
Ответственная библиотека:
BR1.1
ABSTRACT
The ratio of (Na++K+)-ATPase (EC 3.6.1.3.) isoforms with high and low affinity for cardiac glycosides was studied in heart preparations from neonatal, 3-month and 6-month old Wistar rats. Biphasic ouabain inhibition curves of (Na++K+)-ATPase activity indicated that the relative contribution of the high-affinity process decreased from 34 percent at 9 days to 23 percent at 3 months and to 10 percent at 6 months. Scatchard plots for [3H]ouabain binding were curvillinear and indicated that the relative contribution of the high-affinity sites (Kd = 0.1-0.25 µM) decreased by about one-half between 3 months (19-24 percent, N = 2) and 6 months (9-11 percent, N = 2)
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База данных:
LILACS
Основная тема:
Ouabain
/
Sodium-Potassium-Exchanging ATPase
/
Heart Ventricles
/
Isoenzymes
Пределы темы:
Animals
Язык:
En
Журнал:
Braz. j. med. biol. res
Тематика журнала:
BIOLOGIA
/
MEDICINA
Год:
1995
Тип:
Article