DNA extraction from fixed cytogenetic cell suspensions
Genet. mol. res. (Online)
; 6(3): 500-503, 2007. ilus
Article
в En
| LILACS
| ID: lil-498922
Ответственная библиотека:
BR26.1
ABSTRACT
We developed a procedure for DNA extraction from small volumes of fixed cell suspensions previously prepared for conventional cytogenetic analysis. Good quality DNA was isolated with a fast and simple protocol using DNAzol reagent. This provided suitable DNA for various types of molecular analyses, including polymerase chain reaction, restriction fragment length polymorphism, denaturing high-performance liquid chromatography, and direct sequencing. This technique provides sufficient material for such test, which are important for diagnosis of neoplastic diseases in pediatric patients.
Полный текст:
1
База данных:
LILACS
Основная тема:
DNA
/
Genome
/
Cytogenetics
/
Neoplasms
Тип исследования:
Guideline
Пределы темы:
Humans
Язык:
En
Журнал:
Genet. mol. res. (Online)
Тематика журнала:
BIOLOGIA MOLECULAR
/
GENETICA
Год:
2007
Тип:
Article
/
Project document