Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis
Braz. j. microbiol
; 45(1): 225-230, 2014. ilus, tab
Article
в En
| LILACS
| ID: lil-709456
Ответственная библиотека:
BR1.1
ABSTRACT
The initial growth of mycobacteria from 49 samples of cattle and buffalo organs collected in commercial slaughterhouses was compared between modified Middlebrook 7H11 thin layer microcolony culture and Stonebrink medium used in the isolation of Mycobacterium bovis. Aliquots were decontaminated by Petroff's method, processed and cultured in both media. The identity of the acid-fast bacilli stained by Ziehl-Neelsen was confirmed by PCR. Optical microscopy showed that results of the early observation of Mycobacterium bovis colonies in thin layer culture were similar to those obtained in macroscopic observation of the colonies in Stonebrink medium. However, early observation of the colonies enabled early confirmation by PCR, given the shorter time to the visualization of colonies when thin layer culture was used (between the 12nd and 25th day of culture).
Key words
Полный текст:
1
База данных:
LILACS
Основная тема:
Tuberculosis, Bovine
/
Polymerase Chain Reaction
/
Bacteriological Techniques
/
Molecular Diagnostic Techniques
/
Meat
/
Mycobacterium bovis
Тип исследования:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
/
Risk_factors_studies
Пределы темы:
Animals
Язык:
En
Журнал:
Braz. j. microbiol
Тематика журнала:
MICROBIOLOGIA
Год:
2014
Тип:
Article